Cloning and characterization of a calcium-dependent protein kinase from corn roots by Anju Bhatia

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  • Protein kinases.,
  • Corn.,
  • Cloning.

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Statementby Anju Bhatia.
The Physical Object
Paginationviii, 76 leaves, bound :
Number of Pages76
ID Numbers
Open LibraryOL16951826M

Download Cloning and characterization of a calcium-dependent protein kinase from corn roots

The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals.

In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins Cited by: Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development roots, hypocotyls, and leaves were collected from the seedlings.

Flowers, ovules and fibers were harvested from field-grown cotton plants. For fiber collection, flowers were. Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development. Huang QS(1), Wang HY, Gao P, Wang GY, Xia GX. Author information: (1)State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijingby:   The junction domain is the highest conserved region, com- Fig.

1 The full-length cDNA and deduced amino acid sequences of NtCDPK12 (GenBank accession no. GQ). The nucleotides and amino acids are numbered on the left. Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought Cited by: 5.

Cloning and characterization of a calcium-dependent protein kinase from corn roots book In plants, calcium-dependent protein kinases (CDPKs) are key intermediates in calcium-mediated signaling that couple changes in Ca2+ levels to a specific response.

In the present study, we report the high-level soluble expression of calcium-dependent protein kinase1 from Cicer arietinum (CaCDPK1) in Escherichia coli.

The expression of soluble CaCDPK1 was temperature dependent with Cited by: 4. Kinase Assay. A fusion protein was produced by cloning the kinase domain of BIKe, base pairs – (amino acids 1–) in frame with glutathione S-transferase (GST) in the pGEX-4T-3 vector (Amersham Pharmacia Biotech) followed by expression inEscherichia coli.

The fusion protein (GST-BIKeKD) was purified by affinity chromatography using. Plant Physiol. () Characterization of a Calcium-Dependent Protein Kinase from Arachis hypogea (Groundnut) Seeds' Maitrayee DasCupta* Department of Biophysics, University of Delhi South Campus, Benito Juarez Road, New Delhilndia.

Phosphoinositide-3 kinase activity is implicated in diverse cellular responses triggered by mammalian cell surface receptors and in the regulation of protein sorting in yeast.

Receptors with intrinsic and associated tyrosine kinase activity recruit heterodimeric phosphoinositide-3 kinases that consist of p catalytic subunits and p85 adaptor molecules containing Src homology 2 (SH2) domains.

In plants, numerous Ca2+-stimulated protein kinase activities occur through calcium-dependent protein kinases (CDPKs). These novel calcium sensors are likely to be crucial mediators of responses to diverse endogenous and environmental cues.

However, the precise biological function(s) of most CDPKs remains elusive. The Arabidopsis genome is predicted to encode 34 different CDPKs. Molecular Cloning and Characterization of a Novel Protein Kinase with a Catalytic Domain Homologous to Mitogen-activated Protein Kinase Kinase Kinase* Sunny Wang Xuhong We report herein the isolation of a cDNA encoding a novel protein kinase designated MAPKKK5 from a human macrophage library.

The nucleotide sequence predicts that MAPKKK5. Setsuko Komatsu, Guangxiao Yang, Monowar Khan, Haruko Onodera, Seiichi Toki, Masayuki Yamaguchi, Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants, Molecular Genetics and Genomics, /s,6, (), ().

1. Introduction. Calcium (Ca 2+) is a ubiquitous second messenger in both plants and m-dependent protein kinases (CDPKs/CPKs) are a group of Ser/Thr protein kinases that are broadly distributed in plants and some protozoans only and decode Ca 2+ signals (Luan et al., ; Kudla et al., ).CPKs are composed of four characteristic functional domains, which include.

The first calcium-dependent, calmodulin-indepen-dent protein kinase activities were reported in pea (Pisum sativum) extracts 20 years ago (Hetherington and Trewavas, ). Since their initial purification and characterization from soybean (Glycine max; Har-mon et al., ), CDPKs have been identified throughout the plant kingdom from green.

Molecular cloning, characterization and functional analysis of a putative mitogen-activated protein kinase kinase kinase 4 (MEKK4) from blood clam Tegillarca granosa. Author links open overlay panel Guosheng Liu a b 1 Mingliang Chen b 1 Chen Yu a b Wei Wang b Lirong Yang b Zengpeng Li b Weiyi Wang b Jianming Chen b.

CDPK, calcium-dependent protein kinase or calmodulin-like domain protein kinase PCR, polymerase chain reaction. In higher plants, as well as in animals and microorganisms, calcium seems to play a pivotal role as a second messenger by regulating many aspects of cellular signaling [1].

In this report we describe the cloning and functional characterization of a novel serine threonine kinase. This kinase has been termed PKD2, because it was found to be highly homologous to the family of protein kinases comprising PKD/PKCμ and PKCν.

Molecular cloning and characterization of a novel calcium/calmodulin-dependent protein kinase II inhibitor from human dendritic cells. Zhang J(1), Li N, Yu J, Zhang W, Cao X. Author information: (1)Department of Clinical Laboratory, Changzheng Hospital, ShanghaiPeople's Republic of China.

A.J. Robison, R.J. Colbran, in Encyclopedia of Biological Chemistry (Second Edition), Ca 2+ /Calmodulin-Dependent Protein Kinase Kinase. CaMKK, originally characterized as an activating factor from brain extracts, can dramatically increase the activity of CaMKI and CaMKIV when incubated in the presence of Ca 2+ /calmodulin, Mg 2+, and isoforms (α and β) of CaMKK have been.

In plants, the multigene family of calcium-dependent protein kinases (CDPKs) encodes structurally conserved, unimolecular calcium sensor/protein kinase effector proteins. CDPKs are ideal candidates for perceiving intracellular changes in Ca 2+ concentration and translating them into specific phosphorylation events to initiate further downstream.

A calmodulin-binding protein kinase with strong homol- ogy to CaMKII has been cloned from apple (Watillon et al., ), but calcium-dependent, calmodulin-independent CDPKs seem to be the predominant calcium-dependent protein kinases in plants. The other major family in the CaMK group, the SNFl homologs, have been identified in plants.

() Characterization of a Ca 2+ /calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.

Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. Stoyanov B(1), Volinia S, Hanck T, Rubio I, Loubtchenkov M, Malek D, Stoyanova S, Vanhaesebroeck B, Dhand R, Nürnberg B, et al.

Author information: (1)Max Planck Research Unit in Growth Factor Signal Transduction, Medical Faculty, University of Jena, Germany. Ca 2+ /calmodulin-dependent protein kinase II (CaM kinase II or CaMKII) is a serine/threonine-specific protein kinase that is regulated by the Ca 2+ /calmodulin complex.

CaMKII is involved in many signaling cascades and is thought to be an important mediator of learning and memory. CaMKII is also necessary for Ca 2+ homeostasis and reuptake in cardiomyocytes, chloride transport in epithelia.

The protein kinase C (PKC) 1 group is a large family of phospholipid-dependent serine/threonine protein kinases that are involved in a wide variety of cellular processes, including membrane receptor signal transduction, control of gene expression, and cell proliferation and differentiation ().Recent molecular cloning studies have revealed that the PKC family consists of at least 11 distinct.

Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel. [Z Qian, D Okuhara, M K Abe, M R Rosner] PMID Abstract ERK7, a member of the mitogen-activated protein kinase family, has a carboxyl-terminal tail that is required for ERK7 activation, cellular localization, and its.

Introduction. Calcium (Ca 2+) signaling is a highly integrated signaling network that plays a fundamental role in growth, development and stress responses in lic Ca 2+ concentrations change in complex spatio-temporal patterns in response to various stimuli.

In plants, these altered Ca 2+ signatures lead to specific cellular responses including stomatal movement, increased water. A combination of protein kinase A type II (RII) overlay screening, database searches and PCR was used to identify a centrosomal A‐kinase anchoring protein.

A cDNA with an kb open reading frame w. While, the enzymatic activity of CDPK1 remains to be tested, we outline a brief account of Ca 2+-dependent protein kinase activity reported in other algal first report for the presence of a CDPK-like enzyme was performed in by Guo and Roux who partially purified and characterized the enzyme from D.

enzyme, identified using an in-gel kinase assay and shown to be. Human Ca(2+)‐calmodulin (CaM) dependent protein kinase I (CaMKI) encodes a amino acid protein with a calculated M(r) of 41, The kb CaMKI mRNA is expressed in many different human tissues and is the product of a single gene located on human chromosome 3.

Cloning and characterization of the human protein kinase C-eta promoter. Protein kinase C-eta (PKC-eta) is predominantly expressed in epithelial tissue, including lung, intestine, and skin. In skin, PKC-eta expression is limited to keratinocytes in the upper layers of the epidermis.

To investigate regulation of cell type-specific expression. Calcium-dependent protein kinases (CDPKs) are important sensors of Ca+2 flux in plants, which control plant development and responses by regulating downstream components of calcium signaling pathways.

Availability of the whole genome sequence and microarray platform allows investigation of genome-wide organization and expression profile of CDPK genes in rice with a view to. Abstract. Human Ca(2+)-calmodulin (CaM) dependent protein kinase I (CaMKI) encodes a amino acid protein with a calculated M(r) of 41, The kb CaMKI mRNA is expressed in many different human tissues and is the product of a single gene located on human chromosome 3.

Protein kinase CK2 is a ubiquitous Ser/Thr kinase that is highly conserved among eukaryotes; it is a heterotetramer composed of two catalytic (α) and a dimer of regulatory (β) subunits.

Calcium-Dependent Protein Kinase Genes in Corn Roots Two cDNAs encoding Ca-2(+) - Dependent Protein Kinases (CDPKs), Corn Root Protein Kinase 1 and 2 (CRPK 1, CRPK 2) were isolated from the root tip library of corn (Zea mays L., cv.

Merit) and their nucleotide sequences were determined. Deduced amino acid sequences of both the clones have features characteristic of plant CDPKS. The goals of this study were to identify specific mRNAs for isoforms of calmodulin-dependent protein kinase II in chicken forebrain, prepare a cDNA expression library, and perform a sequence analysis of the kinase cDNA.

Specific mRNAs for α- and β-subunits of the kinase were identified in Northern blots. The mRNA for the α-subunit is larger in the chicken than in the rat, and for the β.

The product of the CAMK2A gene is an enzyme that belongs to the serine/threonine-specific protein kinase family, as well as the Ca 2+ /calmodulin-dependent protein kinase II subfamily. Ca 2+ ion signaling is crucial for several aspects of plasticity at glutamatergic synapses.

This enzyme is composed of four different chains: alpha, beta, gamma. When considering Ca 2+ binding effector molecules, it is interesting to note that Plasmodium—like plants—lack orthologs of protein kinase C, but has an expanded family of Ca 2+ dependent protein kinases (CDPKs).

CDPKs combine an amino-terminal serine/threonine kinase domain and a carboxy-terminal calmodulin-like domain, composed of four EF hands, in the same molecule. Calcium is a ubiquitous second messenger that mediates plant responses to developmental and environmental cues.

Calcium‐dependent protein kinases (CDPKs) are key actors of plant signaling that convey calcium signals into physiological responses by phosphorylating various substrates including ion channels, transcription factors and metabolic enzymes.

The β subunit of casein kinase II: cloning of cDNAs from murine and porcine origin and expression of the porcine sequence as a fusion protein. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression(3), DOI: /(91)H.

Tony Hunter. [1] Protein kinase classification. A. Dubrovina, O. Aleynova, K. Kiselev, Influence of overexpression of the true and false alternative transcripts of calcium-dependent protein kinase CPK9 and CPK3a genes on the growth, stress tolerance, and resveratrol content in Vitis amurensis cell cultures, Acta Physiologiae Plantarum, /sy, 38, 3, ().

1 Introduction. The 14‐3‐3 proteins are small highly conserved eukaryotic proteins which have been implicated in regulating multiple cellular enzymes, including protein kinases [2, 13].

14‐3‐3 proteins can bind and modulate the activity of protein kinase C [1, 3] and Raf kinase [11, 14, 22, 30].In addition, they bind wee1 kinase [] and mitogen‐activated protein kinase (MEK) kinases [].Protein kinase C (PKC) translocates from the soluble to the cell particulate fraction on activation.

Intracellular receptors that bind activated PKC in the particulate fraction have been implicated by a number of studies. Previous work identified to kDa proteins in the particulate fraction of heart and brain that bound activated PKC in a specific and saturable manner.Two isoforms of the protein kinase A catalytic subunit, Cα and Cβ, have previously been described in the mouse.

We now report the cloning and characterization of a novel C-related sequence, Cx, from a murine genomic library. Cx is % identical to part of the Cα coding region, but lacks all of the introns present in this gene, suggesting that is arose via retroposition.

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